FC Spartak Trnava was founded on 30 May 1923 through the merger of two local clubs, ŠK Čechie and ČšŠK, into TŠS Trnava, playing on the same city-centre ground it still occupies today. Under communist rule the club underwent a series of enforced name changes, briefly becoming Sokol and then TJ Kovosmalt, tied to the local metal industry, before settling on the name Spartak in 1953.
The club's golden era began on 9 June 1968, when, in front of 20,000 spectators in the rain, Spartak beat Jednota Trenčín 2-0 to win its first Czechoslovak championship, with Jozef Adamec finishing as the league's top scorer. Coach Anton Malatinský, who left for Austria immediately afterwards, had built the foundations of a side that, under his successor Ján Hucko, retained the title the following season and reached the semi-finals of the 1968-69 European Cup, the furthest any Slovak or Czech club has ever advanced in the competition. There, Spartak lost 3-2 on aggregate to Johan Cruyff's Ajax, having also come close to a continental trophy the previous season, finishing runners-up to Red Star Belgrade in the 1967-68 Mitropa Cup. Between 1968 and 1973, the club won five Czechoslovak titles in six seasons, a run that also produced quarter-final appearances in the European Cup in 1973 and 1974, and included players such as Ladislav Kuna and Karol Dobiaš, the latter the only Trnava-developed player in Czechoslovakia's title-winning squad at Euro 1976.
After Czechoslovakia split in 1993, Spartak remained a respected name in Slovak football but had to wait until the 2017-18 season for its first title as an independent Slovak champion, adding domestic cups in 2019, 2022 and 2023 despite periods of financial and organisational instability.
Nicknamed Bíli anjeli ("The White Angels") despite their red-and-black colours, Spartak play at the stadium named in 1998 after Malatinský, whose tactical legacy still anchors the club's identity. Deeply rooted in the city of Trnava, not far from Bratislava, Spartak continues to represent Slovakia in European competition, carried by a fan base whose loyalty has outlasted the club's many ups and downs.